DEVELOPMENT OF A TEXTURE-FORMING FOOD COMPOSITION FORMULATION USING TRANSGLUTAMINASE FOR COOKED SAUSAGE PRODUCTS

Keywords: hydrocolloids, whey, transglutaminase, composite mixture, dynamic viscosity, rheological indicators

Abstract

The development of effective texture-regulating food additives for cooked sausage products requires taking into account their thermal stability to ensure the necessary quality indicators in the production process. To ensure viscosity indicators, a wide class of hydrocolloids is used in the composition of textureforming additives – starches, gums, carboxymethyl cellulose, proteins of animal and plant origin. To form the rheological indicators of systems containing protein, to form a given texture, transferases of a given enzymatic activity are also used. The selection of components of mixtures based on hydrocolloids should take into account the increase in the stability of rheological indicators, in particular dynamic viscosity, to form quality indicators of sausage products. In the studies, potato starch, xanthan gum (E415), carboxymethyl cellulose (E466), konjac gum (E425), leavening agent (E551), dry demineralized whey and transglutaminase enzyme were used to develop a texture-forming food composition. In the process of studying the indicators of 1 % and 2 % solutions of model compositions of mixtures, the effect of the prescription combination of hydrocolloids, whey and transglutaminase on the dynamic viscosity indicators for 2 and 12 hours of storage of the solutions was determined. The effect of the prescription combination of the composition of the mixtures on the change in dynamic viscosity after heating to a temperature of 73°C was studied. This allowed us to assess the thermal stability of the composite mixtures using transglutaminase. It has been established that the use of transglutaminase with enzymatic activity within 90–130 units in an amount of 10–15 % in the composition of texture-forming compositions with whey allows to increase the stability of dynamic viscosity indicators during heat treatment, which simulates the completion of the heating process of cooked sausage products.

References

1. Akbari M., Razavi S. H., Kieliszek M. Recent advances in microbial transglutaminase biosynthesis and its application in the food industry. Trends in Food Science & Technology. 2021. Vol. 110. P. 458–469. URL: https://doi.org/10.1016/j.tifs.2021.02.036.
2. Nature-derived ingredients as sustainable alternatives for tenderizing meat and meat products: An updated review / E. G. Anaduaka et al. Food Biotechnology. 2023. Vol. 37, no. 2. P. 136–165. URL: https://doi.org/10.1080/08905436.2023.2201354.
3. Function of Different Emulsifiers in Spreadable Meat Emulsions: A Systematic Study of Physical Properties / M. Baechle et al. Food Biophysics. 2025. Vol. 20, no. 2. URL: https://doi.org/10.1007/s11483-025-09941-2.
4. Dietary fibres in processed meat: A review on nutritional enhancement, technological effects, sensory implications and consumer perception / M.-M. Ciobanu et al. Foods. 2025. Vol. 14, no. 9. P. 1459. URL: https://doi.org/10.3390/foods14091459.
5. Fadeyi̇bi̇ A. Modeling rheological behavior of beef based on time-dependent deformation and packaging. Gazi University Journal of Science. 2021. URL: https://doi.org/10.35378/gujs.742087.
6. Underlying the interactions in myofibrillar proteins and κ-carrageenan mixed sols as mediated by microbial transglutaminase based on conformational alterations, rheological behavior and molecular docking / Y. Feng та ін. Food Hydrocolloids. 2025. С. 111253. URL: https://doi.org/10.1016/j.foodhyd.2025.111253.
7. Comprehensive review of carrageenan’s multifaceted role in health and food systems / G. Kalsi et al. Discover Food. 2025. Vol. 5, no. 1. URL: https://doi.org/10.1007/s44187-025-00405-7.
8. Influence of functional food composition on the properties of meat mince systems / I. Strashynskiy et al. Eastern-European Journal of Enterprise Technologies. 2016. Vol. 6, no. 11 (84). P. 53–58. URL: https://doi.org/10.15587/1729-4061.2016.86957.
9. Development of combined protein-fat emulsions for sausage and semifinished products with poultry meat / V. Pasichniy et al. Eastern-European Journal of Enterprise Technologies. 2015. Vol. 1, no. 6 (73). P. 32–38. URL: https://doi.org/10.15587/1729-4061.2015.36232.
10. Influence of soybean dietary fiber with varying particle sizes and transglutaminase on soy protein isolate gel / Y. Lv et al. Food Research International. 2022. Vol. 161. P. 111876. URL: https://doi.org/10.1016/j.foodres.2022.111876.
11. Montes L., Rosell C. M., Moreira R. Rheological properties of corn starch gels with the addition of hydroxypropyl methylcellulose of different viscosities. Frontiers in Nutrition. 2022. Vol. 9. URL: https://doi.org/10.3389/fnut.2022.866789.
12. Effects of pre-gelatinized whole Pueraria powder on the properties of duck meat sausages: Insights into gelatinization and molecular interactions / J. Shao et al. Food Chemistry. 2025. P. 146020. URL: https://doi.org/10.1016/j.foodchem.2025.146020.
13. Rudiuk V., Pasichnyi V. Assessment of function-technological and rheological parameters of consistency stabilisers for dairy protein-fat systems for the production of semi-smoked sausages. Technology audit and production reserves. 2023. Vol. 3, no. 3(71). P. 41–45. URL: https://doi.org/10.15587/2706-5448.2023.283465.
14. Biotransformation of collagen-containing meat materials into valuable product / O. Topchii et al. Bioconversion of waste to value-added products / ed. by O. Stabnikova et al. Boca Raton, London, New York, 2023. P. 37–68. URL: https://doi.org/10.1201/9781003329671-2.
15. Effect of transglutaminase on quality characteristics of two-structure cookedsmoked sausage / I. Shevchenko et al. Ukrainian Food Journal. 2025. Vol. 14, no. 4. P. 698–712. URL: https://doi.org/10.24263/2304-974X-2025-14-4-8.
16. Encapsulation of lutein in gelatin type A/B-chitosan systems via tunable chains and bonds from tweens: Thermal stability, rheologic property and food 2D/3D printability / H. Wang et al. Food Research International. 2023. Vol. 173. P. 113392. URL: https://doi.org/10.1016/j.foodres.2023.113392.
17. Вплив білоквмісних композицій на основі колагену на якість ковбасніх виробів / А. І. Українець та ін. Харчова наука і технологія. 2016. № 3. С. 50–55. URL: https://doi.org/10.15673/fst.vl0i3.181.
18. Tetrasodium pyrophosphate promotes light meromyosin crosslinking by microbial transglutaminase / Q. Wang et al. Food Chemistry. 2020. P. 128910. URL: https://doi.org/10.1016/j.foodchem.2020.128910.
19. Agar-based composite emulsion gel as a pork fat substitute in sausages: Understanding meat batter stabilization mechanisms based on fat sources / M. Woo et al. International Journal of Biological Macromolecules. 2025. Vol. 318. P. 144851. URL: https://doi.org/10.1016/j.ijbiomac.2025.144851.
20. Гармаш Д., Пасічний В. Вплив режимів термічної обробки на характеристики продуктів із м’яса птиці з використанням цільової ферментації. Вісник Національного технічного університету «ХПІ». Серія: Нові рішення у сучасних технологіях. 2021. № 3(5). С. 40–44. URL: https://doi.org/10.20998/2413-4295.2020.01.06.
21. Effects of ultrasound-assisted extraction and transglutaminase treatment on the physicochemical properties of protein from Stropharia rugosoannulata / L. Yang et al. Ultrasonics Sonochemistry. 2025. Vol. 122. P. 107637. URL: https://doi.org/10.1016/j.ultsonch.2025.107637.
22. Youssef M. K., Barbut S. Effects of two types of soy protein isolates, native and preheated whey protein isolates on emulsified meat batters prepared at different protein levels. Meat Science. 2011. Vol. 87, no. 1. P. 54–60. URL: https://doi.org/10.1016/j.meatsci.2010.09.002.
23. Effect of carboxymethyl cellulose (CMC) on some physico-chemical and mechanical properties of unrinsed surimi gels / C. Zhang et al. Lwt. 2023. P. 114653. URL: https://doi.org/10.1016/j.lwt.2023.114653.
Published
2026-05-08
Section
MODERN DIRECTIONS FOR THE DEVELOPMENT OF FOOD TECHNOLOGY