DEVELOPMENT OF COMBINED SMOOTHIES AND EVALUATION OF THEIR PROPERTIES
Abstract
The growing consumer demand for healthy foods and the increasing popularity of the functional nutrition concept have intensified the need to develop innovative beverages with enhanced nutritional and biological value. Plant-based smoothies have attracted considerable attention owing to their potential to combine fruits, vegetables, and cereal ingredients into balanced formulations rich in essential macro- and micronutrients while providing desirable sensory properties. The objective of this study was to develop formulations of combined plant-based smoothies using soy beverage as a liquid base and to evaluate their sensory and physicochemical characteristics. Several smoothie formulations containing vegetables, fruits, cereal ingredients, and soy beverage were developed and experimentally evaluated. The protein, total solids, ash, calcium, magnesium, pH, and titratable acidity of the samples were determined, and their sensory properties were assessed using an integrated quality index. The obtained results demonstrated that the formulations containing soy beverage, zucchini, beetroot, pumpkin, spinach, apple, oat flakes, and buckwheat flakes exhibited the most favorable quality characteristics. These samples contained 3.20–3.27 g/100 g of protein, 1282–1362 mg/L of calcium, 153–168 mg/L of magnesium, and 10.81–10.96% total solids, with pH values ranging from 6.68 to 6.68. The protein content of the developed smoothies exceeded that typically reported for conventional fruit and vegetable smoothies, indicating the nutritional advantages of incorporating soy beverage and cereal ingredients into the formulation. The integrated quality index of 0.830–0.831 confirmed the high sensory acceptability of the recommended smoothie formulations. The scientific novelty of the study lies in the development of original formulations of combined plant-based smoothies enriched with soy beverage and powdered oat and buckwheat flakes, as well as in the comprehensive evaluation of their physicochemical and sensory characteristics. The practical significance of the study is associated with the possibility of implementing the proposed formulations in the production of functional beverages with improved nutritional value, increased protein content, and enhanced mineral composition. The results confirm that the use of soy beverage as a liquid base in combination with cereal ingredients is an effective approach to improving both the nutritional quality and consumer properties of smoothies. The developed smoothie formulations can be recommended for the production of functional plant-based beverages intended for consumers seeking nutritionally balanced products. Future research should focus on evaluating the effects of different plant-based beverages used as liquid bases on smoothie quality parameters, storage stability, and consumer acceptance, as well as on optimizing formulations to obtain products with targeted nutritional and functional properties.
References
2. Yusuf E. H., Wojdyło A., Bourbon A. I., Nowicka P. Fruit–carrot-based smoothies as innovative products with a complex matrix of bioactive compounds effected on activities of selected digestive enzymes and cholinesterases in vitro. Antioxidants. 2023. Vol. 12(4). P. 917. URL: https://doi.org/10.3390/antiox12040917.
3. Da Silva-Mojón L., Pérez-Lamela C., Falqué-López E. Smoothies marketed in Spain: Are they complying with labeling legislation? Nutrients. 2023. Vol. 15(20). P. 4426. URL: https://doi.org/10.3390/nu15204426.
4. Ashraf H., Khalid S., Khalid W., Nadeem M., Tahir M.U. Vegetables’ juices, blends, and smoothies: Preparation and processing. In : Iqbal Z., Anjum M.A., Ercisli S., Rauf S. (eds), Sustainable and Innovative Vegetable Production in times of Climate Change. Singapore : Springer, 2025. URL: https://doi.org/10.1007/978-981-96-6283-8_28.
5. Neelissen J., Leanderson P., Jonasson L., Chung R. W. S. The effects of dairy and plant-based liquid components on lutein liberation in spinach smoothies. Nutrients. 2023. Vol. 15(3). P. 779. URL: https://doi.org/10.3390/nu15030779.
6. Jiménez-Monreal A. M., Cedeño-Pinos C., Bañón S., Muñoz I., Guardia M. D., Tahori N., Martínez-Tomé M. Effect of in vitro gastrointestinal digestion on the antioxidant properties of fruit and vegetable powdered smoothies reinforced with WPC80. LWT. 2025. Vol. 215. P. 117301. URL: https://doi.org/10.1016/j.lwt.2024.117301.
7. Bhullar S. Health associations of various fruit forms: solid fruits, juices, and smoothies. Frontiers in Nutrition. 2025. Vol. 12. P. 1626179. URL: https://doi.org/10.3389/fnut.2025.1626179.
8. Razola-Díaz M. d. C., Guerra-Hernández E. J., García-Villanova B., Verardo V. New insight on phenolic composition and evaluation of the vitamin C and nutritional value of smoothies sold on the Spanish market. Molecules. 2022. Vol. 27(23). P. 8229. URL: https://doi.org/10.3390/molecules27238229.
9. Michael J. A., Olatuyi V. T., Adelu A. O., Falodun A. I., Ogungbenro G. M., Nwanna E. E., Elekofehinti O.O., Badejo A. A. Antioxidant activities of developed natural smoothies and their effects on blood glucose levels and expression of pro-inflammatory genes in streptozotocin-induced diabetic rats. Food Production, Processing and Nutrition. 2024. Vol. 6(1). P. 78. URL: https://doi.org/10.1186/s43014-024-00249-1.
10. Donda Zbinden M., Schmidt M., Vignatti C. I., Pirovani M. É., Böhm V. High-pressure processing of fruit smoothies enriched with dietary fiber from carrot discards: Effects on the contents and bioaccessibilities of carotenoids and vitamin E. Molecules. 2024. Vol. 29(6). P. 1259. URL: https://doi.org/10.3390/molecules29061259.
11. Van de Velde F., Vignatti C., Méndez-Galarraga M. P., Gomila M., Fenoglio C., Zbinden M. D., Pirovani M. É. Intestinal and colonic bioaccessibility of phenolic compounds from fruit smoothies as affected by the thermal processing and the storage conditions. Food Research International. 2022. Vol. 155. P. 111086. URL: https://doi.org/10.1016/j.foodres.2022.111086.
12. Šilha D., Syrová P., Syrová L., Janečková J. Smoothie drinks: Possible source of resistant and biofilm-forming microorganisms. Foods. 2022. Vol. 11(24). P. 4039. URL: https://doi.org/10.3390/foods11244039.
13. De B., Shrivastav A., Das T., Goswami T. K. Physicochemical and nutritional assessment of soy milk and soymilk products and comparative evaluation of their effects on blood gluco-lipid profile. Applied Food Research. 2022. Vol. 2(2). P. 100146. URL: https://doi.org/10.1016/j.afres.2022.100146.
14. Mollakhalili-Meybodi N., Arab M., Zare L. Harmful compounds of soy milk: characterization and reduction strategies. Journal of Food Science and Technology. 2022. Vol. 59(10). P. 3723–3732. URL: https://doi.org/10.1007/s13197-021-05249-4.
15. Sargautis D., Kince T. Effect of enzymatic pre-treatment on oat flakes protein recovery and properties. Foods. 2023. Vol. 12(5). P. 965. URL: https://doi.org/10.3390/foods12050965.
16. Reiners S., Hebestreit S., Wedekind L., Kiehntopf M., Klink A., Rummler S., Glei M., Lorkowski S., Schlörmann W., Dawczynski C. Effect of a regular consumption of traditional and roasted oat and barley flakes on blood lipids and glucose metabolism – A randomized crossover trial. Frontiers in Nutrition. 2023. Vol. 10. P. 1095245. URL: https://doi.org/10.3389/fnut.2023.1095245.
17. Sonawane S., Shams R., Dash K. K., Patil V., Pandey V. K., Dar A. H. Nutritional profile, bioactive properties and potential health benefits of buckwheat: A review. EFood. 2024. Vol. 5(4). P. 171. URL: https://doi.org/10.1002/efd2.171.
18. Silva G. d. F., Peixoto T. D. C., Ferreira Neto M., Souto A. G. d. L., Rodrigues Filho R. A., Araújo K. F. d. P. Cavalcante J. S. J., Pereira K. T. O., Moreira R. C. L., Fernandes P. D., Dias N. d. S., Rocha J. L. A., Melo A. S. d., Silva A. Á. d., Sá F. V. d. S. Effect of nitrogen sources on the phenological phases of Italian zucchini under salt stress. Agriculture. 2026. Vol. 16(1). P. 62. URL: https://doi.org/10.3390/agriculture16010062.
19. Jhanani G. K., Alsalhi M. S., Shanmuganathan R. As assessment of shelf life increasing competence of pectin (Zucchini) based edible coating on tomatoes. Environmental Research. 2024. Vol. 258. P. 119368. URL: https://doi.org/10.1016/j.envres.2024.119368.
20. Bangar S. P., Sharma N., Sanwal N., Lorenzo J. M., Sahu J. K. Bioactive potential of beetroot (Beta vulgaris). Food Research International. 2022. Vol. 158. P. 111556. URL: https://doi.org/10.1016/j.foodres.2022.111556.
21. Stoica F., Râpeanu G., Rațu R. N., Stănciuc N., Croitoru C., Țopa D., Jităreanu G. Red beetroot and its by-products: A comprehensive review of phytochemicals, extraction methods, health benefits, and applications. Agriculture. 2025. Vol. 15(3). P. 270. URL: https://doi.org/10.3390/agriculture15030270.
22. Gavril R. N., Stoica F., Lipșa F. D., Constantin O. E., Stănciuc N., Aprodu I., Râpeanu G. Pumpkin and pumpkin by-products: A comprehensive overview of phytochemicals, extraction, health benefits, and food applications. Foods. 2024. Vol. 13(17). P. 2694. URL: https://doi.org/10.3390/foods13172694.
23. Hussain A., Kausar T., Sehar S., Sarwar A., Quddoos M. Y., Aslam J., Liaqat A., Siddique T., Ul An Q., Kauser S., Rehman A., Nisar R. A review on biochemical constituents of pumpkin and their role as pharma foods; a key strategy to improve health in post COVID-19 period. Food Production, Processing and Nutrition. 2023. Vol. 5(1). P. 22. URL: https://doi.org/10.1186/s43014-023-00138-z.
24. Sarma U., TR B. Dietary phytonutrients in common green leafy vegetables and the significant role of processing techniques on spinach: A review. Food Production, Processing and Nutrition. 2024. Vol. 6. P. 10. URL: https://doi.org/10.1186/s43014-023-00192-7.
25. Naseem A., Akhtar S., Ismail T., Qamar M., Sattar D.-e.-s., Saeed W., Esatbeyoglu T., Bartkiene E., Rocha J. M. Effect of growth stages and lactic acid fermentation on anti-nutrients and nutritional attributes of spinach (Spinacia oleracea). Microorganisms. 2023. Vol. 11(9). P. 2343. URL: https://doi.org/10.3390/microorganisms11092343.
26. Arnold M., Gramza-Michalowska A. Recent development on the chemical composition and phenolic extraction methods of apple (Malus domestica) – A review. Food and Bioprocess Technology. 2024. Vol. 17. P. 2519–2560. URL: https://doi.org/10.1007/s11947-023-03208-9.
27. Asma U., Morozova K., Ferrentino G., Scampicchio M. Apples and apple by-products: Antioxidant properties and food applications. Antioxidants. 2023. Vol. 12(7). P. 1456. URL: https://doi.org/10.3390/antiox12071456.

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