THE POSSIBILITIES OF AGGLOMERATION OF RIBOFLAVIN BY THE MUTANTS OF СANDIDA QUILLERMONDII YEAST

Abstract

At present stage the resolution of problem of increased synthesis of vitamins, including riboflavin, is impossible without a detailed study of the mechanisms of regulation of their biosynthesis. The article highlights the possibility of obtaining mutants resistant to the action of 8-azaguanine and the study of their flavinogenic activity. It was found, that obtained mutants are not able to desaminate guanine and its analogues, i.e. they retained the block of guanindesaminase. Using mutants with impaired regulation of IMF-dehydrogenase, it was investigated that increasing the fund of guanylic compounds in the cell contributes to the accumulation of large amounts of riboflavin while growing yeast in the environment with a high iron content. The study of mutants resistant to the action of 8-azaguanine supports the hypothesis about the nature of purine predecessor of riboflavin and open the perspective of using regulatory mutants to obtain highly active vitamin producers.